Real Research Single cell cloning in suspension
LifeGel for 3D cell culture consists of multi-well plates pre-coated with LifeGel, available with either cell culture medium (RPMI, DMEM-HG, DMEM-LG, DMEM/F-12, alpha-MEM, custom) or in PBS. This innovative platform provides a ready-to-use solution for advanced 3D cell culture applications, enabling more physiologically relevant cell growth and research.
When you want to avoid cross-contamination from old cell lines, animal cells or other DNA.
APPLICATION
Cell line development
Gene therapy
Production of recombinant protein and monoclonal antibody
Drug screening
Gene functional studies
CRISPR-cas9, ZFNs, TALENs
Step 1
You choose your cell line (adherent or non-adherent) and send it to us. If necessary, we will adapt your cell line to suspension growth and find the right cell culture media which will be later applicable for GMP manufacturing.
Step 2
Single clone selection phase: Your cell line now adapted to suspension growth will receive additional supplements for single clone selection. At this stage you have to decide how many cells you want to attempt the selection with – we suggest 1000 single cells to give a high chance of obtaining good working clones.
We are using our own proprietary method of establishing clones from single cells.
Step 3
Obtaining the necessary number of cells from clones – increasing the scale of culture, and reduction of cell selection media to a growth media.
Shipment of the cells.
Why us?
Stable cell line production
3 banks of 106 cells each
Duration: usually 6-9 months but varies depending on the client’s exact requirements